Measurement of Gly m Bd 30K, a major soybean allergen, in soybean products by a sandwich enzyme-linked immunosorbent assay

Biosci Biotechnol Biochem. 1995 Jan;59(1):150-1. doi: 10.1271/bbb.59.150.

Abstract

By a sandwich enzyme-linked immunosorbent assay, a soybean major allergen, Gly m Bd 30K, in soybean products was measured. The allergen occurred at high concentrations in soy milk, tofu, kori-dofu, and yuba, but its content in kinako was small. No allergen was found in fermented foods such as miso, shoyu, and natto. The allergen was clearly shown to occur in meat balls, beef croquettes, and fried chicken that contained soybean protein isolate.

MeSH terms

  • Allergens / analysis*
  • Animals
  • Dietary Proteins / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Glycine max / chemistry*
  • Glycine max / immunology
  • Immunoblotting
  • Plant Proteins, Dietary / analysis*
  • Plant Proteins, Dietary / chemistry
  • Plant Proteins, Dietary / immunology
  • Soybean Proteins

Substances

  • Allergens
  • Dietary Proteins
  • Plant Proteins, Dietary
  • Soybean Proteins