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Biosci Biotechnol Biochem. 1994 Oct;58(10):1911-3.

Prolonged cell-free protein synthesis in a batch system using wheat germ extract.

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Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Japan.


Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 micrograms dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.

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