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Biotechnology (N Y). 1993 Dec;11(13):1574-8.

Large-scale production of Vibrio cholerae toxin B subunit for use in oral vaccines.

Author information

1
University of Göteborg, Dept. of Medical Microbiology and Immunology, Sweden.

Abstract

By systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin B (CTB) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of CTB approaching one gram per liter. The CTB protein which was secreted to > 95%, could readily be purified from the growth medium of a V. cholerae production strain and was shown to be immunologically indistinguishable from previously used vaccine preparations of native or recombinant CTB.

PMID:
7764248
DOI:
10.1038/nbt1293-1574
[Indexed for MEDLINE]

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