Format

Send to

Choose Destination
Biochem Biophys Res Commun. 1995 May 25;210(3):695-702.

Metabolism of [3H]farnesol to cholesterol and cholesterogenic intermediates in the living rat eye.

Author information

1
Anheuser-Busch Eye Institute, Saint Louis University School of Medicine, MO 63104, USA.

Abstract

Adult rats were injected intravitreally with all-trans [1-3H]farnesol, with or without co-injection of the squalene epoxidase inhibitor NB-598. Retinas were isolated 16 h later and their lipids were extracted, saponified, and analyzed by radio-HPLC. Most (> or = 90%) of the nonsaponifiable radioactivity was recovered as unmetabolized [3H]farnesol; however, about 6-8% of the radioactivity in control retinas exhibited the chromatographic behavior of sterols, including cholesterol. Unlike the controls, the NB-598-treated retinas exhibited substantial accumulation of both [3H]squalene and squalene mass. Calculations indicate that most of the squalene mass was derived from metabolism of endogenous precursors, with an in vivo biosynthetic rate of 46 +/- 17.5 pmol/retina/h. Retinas from eyes injected with all-trans [1-3H]geranylgeraniol yielded only the unmetabolized precursor in the nonsaponifiable extracts. These results suggest that farnesol can be "activated" in vivo (presumably to the corresponding allylic pyrophosphate) in the retina and subsequently metabolized to sterols and sterol precursors.

PMID:
7763243
DOI:
10.1006/bbrc.1995.1715
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center