Format

Send to

Choose Destination
Nucleic Acids Res. 1995 Apr 25;23(8):1350-3.

Correlation of GC content with replication timing and repair mechanisms in weakly expressed E.coli genes.

Author information

1
Laboratoire de Mutagen├Ęse, Institut Jacques Monod, Paris, France.

Abstract

Regional variations of DNA GC content are observed in species as different as S.cerevisiae and humans. In vertebrates and yeast they are correlated with replication timing; late replicating chromosomal regions are more AT-rich than early replicating regions. We show here that gene composition in E.coli also has long range variations which are similarly correlated with replication timing. We suggest that the enrichment in AT base pairs in late replicating DNA reflects differences in DNA repair modes. These sequences, which are in single copy for a greater part of the cell cycle than origin-linked genes, have less opportunity to engage in repair via homologous recombination and therefore may resort more often to translesion synthesis involving the misincorporation of adenine opposite modified nucleotides.

PMID:
7753625
PMCID:
PMC306860
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center