Abstract
The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique. The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial / analysis
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology*
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Base Sequence
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Codon
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Enzyme-Linked Immunosorbent Assay / methods*
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Escherichia coli / genetics
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Gene Expression
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Molecular Sequence Data
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Mycoplasma / genetics
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Mycoplasma / immunology*
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Pneumonia of Swine, Mycoplasmal / diagnosis
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Pneumonia of Swine, Mycoplasmal / genetics
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Pneumonia of Swine, Mycoplasmal / immunology
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Pneumonia of Swine, Mycoplasmal / veterinary*
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Point Mutation
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Recombinant Proteins / immunology
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Swine
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Swine Diseases / diagnosis*
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Swine Diseases / genetics
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Swine Diseases / immunology
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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Codon
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Recombinant Proteins
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surface antigen p46, Mycoplasma