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Braz J Med Biol Res. 1994 Aug;27(8):1829-38.

Molecular cloning and characterization of a novel kallikrein transcript in colon and its distribution in human tissues.

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Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA.


1. We have cloned and characterized a new species of kallikrein cDNA from a human colon cDNA library. The new kallikrein cDNA clone contains a part of intron 2 of the tissue kallikrein gene which is spliced to the remaining exon sequences. It does not contain exons 1 and 2. 2. An in-frame open reading frame is present in the new kallikrein cDNA allowing translation of a 216-amino acid product. The intron-containing kallikrein transcript was detected in salivary glands, pancreas, kidney, colon, prostate gland, testis, spleen, and lung by reverse-transcription/polymerase chain reaction followed by Southern blot analysis using an intron-containing kallikrein-specific oligonucleotide probe. 3. The results indicate that the new species of kallikrein may be processed by alternative splicing or arises from a different transcription initiation site.

[Indexed for MEDLINE]

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