Abstract
Expression of CFA/I fimbriae of Escherichia coli requires the transcriptional activator CfaD. The mechanism by which CfaD activates the CFA/I promoter is to overcome the repression by H-NS, one of the histone-like proteins in E coli. This study addresses the question of which sequences in the promoter region of CFA/I interact with CfaD and H-NS. In order to determine this, deletion mutants of the CFA/I promoter were constructed and cloned upstream of the promoterless lacZ gene. The effect of CfaD and H-NS on the expression of these constructs was determined.
MeSH terms
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Antigens, Bacterial / genetics*
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Proteins / genetics*
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Base Sequence
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Chromosome Deletion
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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Escherichia coli / genetics*
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Escherichia coli / immunology
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Fimbriae Proteins*
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Histones / genetics
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Molecular Sequence Data
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Mutation
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Pili, Sex / genetics*
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Promoter Regions, Genetic*
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Repressor Proteins / genetics
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Transcription, Genetic*
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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DNA-Binding Proteins
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H-NS protein, bacteria
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Histones
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Repressor Proteins
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colonization factor antigens
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Fimbriae Proteins