Regions of the CFA/I promoter involved in the activation by the transcriptional activator CfaD and repression by the histone-like protein H-NS

Biochimie. 1994;76(10-11):1052-4. doi: 10.1016/0300-9084(94)90029-9.

Abstract

Expression of CFA/I fimbriae of Escherichia coli requires the transcriptional activator CfaD. The mechanism by which CfaD activates the CFA/I promoter is to overcome the repression by H-NS, one of the histone-like proteins in E coli. This study addresses the question of which sequences in the promoter region of CFA/I interact with CfaD and H-NS. In order to determine this, deletion mutants of the CFA/I promoter were constructed and cloned upstream of the promoterless lacZ gene. The effect of CfaD and H-NS on the expression of these constructs was determined.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial / genetics*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / immunology
  • Fimbriae Proteins*
  • Histones / genetics
  • Molecular Sequence Data
  • Mutation
  • Pili, Sex / genetics*
  • Promoter Regions, Genetic*
  • Repressor Proteins / genetics
  • Transcription, Genetic*

Substances

  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA-Binding Proteins
  • H-NS protein, bacteria
  • Histones
  • Repressor Proteins
  • colonization factor antigens
  • Fimbriae Proteins