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FEMS Microbiol Lett. 1995 Apr 15;128(1):95-100.

Cloning of a DNA fragment encoding part of a 70-kDa heat shock protein of Candida albicans.

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1
Departament de Microbiología, Facultat de Farmacia, Universitat de València, Spain.

Abstract

Immunoscreening of a mycelial expression library with polyclonal antibodies raised against mycelial cell wall resulted in the detection of a cDNA encoding a heat shock protein of Candida albicans. Sequence analysis of a 0.8-kb cDNA subclone, 2M-1, revealed an open reading frame encoding 244 amino acids. Southern blot analysis with this fragment as a probe demonstrated hybridization to C. albicans DNA. Northern analysis showed a substantial increase in 2M RNA expression levels after cells were subjected to heat shock. Western blot analysis with 2M monospecific antibodies recognized a 70-kDa protein which was present in membrane particles and cytosolic fractions.

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