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Virus Genes. 1995 Jan;9(2):105-12.

Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities.

Author information

1
Division of Tropical Medicine, Kanazawa Medical University, Ishikawa, Japan.

Abstract

Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 in Escherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.

PMID:
7732656
DOI:
10.1007/bf01702653
[Indexed for MEDLINE]

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