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Oncogene. 1995 Apr 20;10(8):1607-14.

Deregulation of c-abl mediated cell growth after retroviral transfer and expression of antisense sequences.

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Fels Institute for Cancer Research and Molecular Biology, Department of Biochemistry, Temple University, Philadelphia, Pennsylvania 19140, USA.


To determine the role of c-abl during cell growth, we constructed a retrovirus vector alpha A, capable of expressing an antisense RNA directed against the abl mRNA. Based on v-abl-mediated 3T3 transformation assay, we showed that the number of transformed foci was reduced 50-94% when alpha A-infected 3T3 cells were superinfected with A-MuLV. Up to a 100% of inhibition could be observed when the time of infection was lengthened. Introduction of the antisense sequence into NIH3T3 cells resulted in reduction of growth rate. These cells entered into S phase from G1 phase of the cell cycle earlier in time than untransduced cells. Thus c-abl serves as a checkpoint during G1/S transition in the cell cycle, and its reduction resulted in deregulation of cell growth.

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