Abstract
Priming of naive CD4+ T cells to Ag requires an antigen-presenting cell (APC) that can take up the Ag and present peptide bound to MHC class II molecules. We have used both in vivo and in vitro approaches to demonstrate that the APC used to prime naive CD4+ T cells depends on the initial form in which an Ag is administered. Although Ag delivered as a peptide was presented most efficiently to CD4+ T cells by DC, these APC were poor at priming to a protein form of the same Ag. In contrast, the presence of B cells was a requisite for priming to protein Ag.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology*
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B-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / immunology*
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Cytochrome c Group / immunology
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Dendritic Cells / immunology
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Histocompatibility Antigens Class II / genetics
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Lymphocyte Activation / immunology*
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Mice
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Mice, Inbred Strains
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Mice, Knockout
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Mice, Transgenic
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Peptide Fragments / immunology*
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Proteins / immunology*
Substances
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Cytochrome c Group
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Histocompatibility Antigens Class II
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I-E-antigen
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Peptide Fragments
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Proteins