Abstract
Pyoverdin production by Pseudomonas aeruginosa strain 7NSK2 was induced by Zn(II) in the presence of iron. A mutant was isolated in which Zn(II) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases. An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF. The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Nucleotidyltransferases / genetics*
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DNA Nucleotidyltransferases / metabolism*
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Escherichia coli Proteins*
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Genes, Bacterial
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Genetic Complementation Test
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Integrases*
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Iron / metabolism
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Molecular Sequence Data
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Mutation
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Oligopeptides*
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Open Reading Frames
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Phenotype
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Pigments, Biological / biosynthesis*
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / metabolism*
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Recombinases
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Restriction Mapping
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Sequence Homology, Amino Acid
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Zinc / pharmacology
Substances
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DNA, Bacterial
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Escherichia coli Proteins
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Oligopeptides
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Pigments, Biological
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Recombinases
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XerC protein, E coli
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pyoverdin
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Iron
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DNA Nucleotidyltransferases
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Integrases
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integron integrase IntI1
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Zinc