The sss gene product, which affects pyoverdin production in Pseudomonas aeruginosa 7NSK2, is a site-specific recombinase

Mol Microbiol. 1994 Dec;14(5):1011-20. doi: 10.1111/j.1365-2958.1994.tb01335.x.

Abstract

Pyoverdin production by Pseudomonas aeruginosa strain 7NSK2 was induced by Zn(II) in the presence of iron. A mutant was isolated in which Zn(II) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases. An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF. The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Nucleotidyltransferases / genetics*
  • DNA Nucleotidyltransferases / metabolism*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Genes, Bacterial
  • Genetic Complementation Test
  • Integrases*
  • Iron / metabolism
  • Molecular Sequence Data
  • Mutation
  • Oligopeptides*
  • Open Reading Frames
  • Phenotype
  • Pigments, Biological / biosynthesis*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / metabolism*
  • Recombinases
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Zinc / pharmacology

Substances

  • DNA, Bacterial
  • Escherichia coli Proteins
  • Oligopeptides
  • Pigments, Biological
  • Recombinases
  • XerC protein, E coli
  • pyoverdin
  • Iron
  • DNA Nucleotidyltransferases
  • Integrases
  • integron integrase IntI1
  • Zinc

Associated data

  • GENBANK/X78478