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Anal Biochem. 1995 Jan 1;224(1):28-31.

Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose.

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1
Department of Animal Science, Faculty of Agriculture, Hokkaido University, Japan.

Abstract

We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

PMID:
7710083
DOI:
10.1006/abio.1995.1004
[Indexed for MEDLINE]
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