Send to

Choose Destination
Immun Infekt. 1995 Feb;23(1):4-8.

[Theory and practice of macrorestriction analysis for the clonal analysis of pathogens].

[Article in German]

Author information

Klinische Forschergruppe, Medizinischen Hochschule Hannover.


The macrorestriction analysis of bacterial genomes is a method that is generally applicable to the typing of bacteria. The chromosome is cleaved with a restriction endonuclease that cuts infrequently and subsequently separated by pulsed-field gel electrophoresis. The fragment pattern defines the genotype of the strain. The relatedness of strains is evaluated from the similarity of the fragment patterns. The quantitative comparison of fragment patterns allows the definition of the infrasubspecific rank of a clone. Macrorestriction analysis is a sensitive and specific method to trace the origin and spread of infections and to analyse the clonal structure of bacterial populations.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center