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Anal Biochem. 1993 Feb 15;209(1):104-8.

Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose.

Author information

1
European Institute for Peptide Research, LTI-CNRS URA 203, University of Rouen, Mont Saint Aignan, France.

Abstract

Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.

PMID:
7682035
[Indexed for MEDLINE]

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