Adhesion molecules involved in protein kinase A- and C-dependent lymphocyte adherence to microvascular endothelial cells

Scand J Immunol. 1993 Mar;37(3):282-8. doi: 10.1111/j.1365-3083.1993.tb02555.x.

Abstract

A twofold increase in lymphocyte adherence to rat microvascular endothelial cells (EC) was achieved by incubating EC for 4 h with IL-1 alpha or dibutyryl-cAMP (stimulators of protein kinase A, PKA) and PMA (stimulator of protein kinase C, PKC). Monoclonal antibodies anti-CD11a, anti-CD18 (LFA-1) and anti-CD49d (VLA-4 alpha) significantly inhibited the increased lymphocyte binding to IL-1 alpha-induced EC, anti-CD18 and to a lesser extent anti-CD11a and anti-CD49d to dibutyryl-cAMP-induced EC, whereas only anti-CD11a and anti-CD18 monoclonal antibodies inhibited PMA-induced lymphocyte binding. These findings suggest that stimulation of PKA and PKC induces lymphocyte binding to EC via different adhesion molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / immunology
  • Bucladesine / metabolism
  • CD18 Antigens
  • Cell Adhesion / drug effects*
  • Cell Adhesion Molecules / metabolism*
  • Endothelium, Vascular / cytology*
  • In Vitro Techniques
  • Intercellular Adhesion Molecule-1
  • Interleukin-1 / pharmacology
  • Lymphocyte Function-Associated Antigen-1 / immunology
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Lymphocytes / cytology*
  • Protein Kinase C / physiology*
  • Protein Kinases / physiology*
  • Rats
  • Receptors, Very Late Antigen / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Vascular Cell Adhesion Molecule-1

Substances

  • Antigens, CD
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Interleukin-1
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Very Late Antigen
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1
  • Bucladesine
  • Protein Kinases
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate