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Gastroenterology. 1993 Mar;104(3):759-71.

Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats.

Author information

1
Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport.

Abstract

BACKGROUND:

The objective of this study was to characterize the acute and chronic inflammation induced by the intramural injection of peptidoglycan-polysaccharide (PG-PS) into the distal colon of genetically susceptible rats.

METHODS:

Blood-to-lumen clearance of 51Cr-ethylenediaminetetraacetic acid, colonic myeloperoxidase activity, colon weight, and plasma nitrite and nitrate levels were determined to quantitate colonic mucosal injury, inflammation, and nitric oxide (NO) production, respectively.

RESULTS:

Intramural injection of PG-PS into the distal colon produced a local biphasic inflammatory response composed of an acute episode 3 days after injection; this was followed by a spontaneous reactivation of chronic granulomatous colitis manifested by colonic thickening, adhesions, and infiltration of the submucosa and muscularis propria with macrophages, neutrophils, and lymphocytes at 3-4 weeks. Mucosal ulcers were evident only at 3 weeks, but hepatic nodules, splenic necrosis, and arthritis were evident at both 3 and 4 weeks after PG-PS injection. PG-PS produced significant increases in colonic mucosal permeability, myeloperoxidase activity, and plasma nitrite and nitrate levels at 3 weeks postinjection compared with controls. PG-PS stimulated the production of nitrite by elicited peritoneal macrophages and neutrophils in vitro.

CONCLUSIONS:

PG-PS produces a chronic granulomatous colitis in rats; this colitis is characterized by enhanced NO production.

PMID:
7680014
DOI:
10.1016/0016-5085(93)91011-6
[Indexed for MEDLINE]

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