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Plant Mol Biol. 1993 Jan;21(2):205-11.

Efficient splicing of an AU-rich antisense intron sequence.

Author information

1
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Dundee, UK.

Abstract

For successful splicing in dicot plants the only recognised intron requirements are 5' and 3' splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.

PMID:
7678764
DOI:
10.1007/bf00019937
[Indexed for MEDLINE]

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