Isolation of cDNAs for mouse phenol and bilirubin UDP-glucuronosyltransferases and mapping of the mouse gene for phenol UDP-glucuronosyltransferase (Ugtla1) to chromosome 1 by restriction fragment length variations

Biochem Genet. 1995 Apr;33(3-4):111-22. doi: 10.1007/BF00557949.

Abstract

The mouse gene for phenol UDP-glucuronosyltransferase (UDPGT; Ugtla1) was mapped at 42 cM on chromosome 1, a position identical to that of the gene for bilirubin UDPGT (Ugtla1), from linkage analysis of a three-point cross test with Idh-1, En-1, and Ugtla1 as marker genes. The cDNAs for mouse phenol and bilirubin UDPGTs, isolated after amplification by PCR, shared an identical 3'-half region. Our results strongly suggest that mouse bilirubin and phenol UDPGTs are expressed from a single gene and involve alternative splicing events. We also detected duplication of the gene for phenol UDPGT in all mouse strains examined with the exception of MOL-MIT and SUB-SHH.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 1
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Expression
  • Genetic Linkage
  • Glucuronosyltransferase / chemistry
  • Glucuronosyltransferase / genetics*
  • Humans
  • In Situ Hybridization
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred NOD
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • RNA Splicing
  • RNA, Messenger
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • RNA, Messenger
  • bilirubin glucuronoside glucuronosyltransferase
  • Glucuronosyltransferase

Associated data

  • GENBANK/D50706
  • GENBANK/D50707