Abstract
The mouse gene for phenol UDP-glucuronosyltransferase (UDPGT; Ugtla1) was mapped at 42 cM on chromosome 1, a position identical to that of the gene for bilirubin UDPGT (Ugtla1), from linkage analysis of a three-point cross test with Idh-1, En-1, and Ugtla1 as marker genes. The cDNAs for mouse phenol and bilirubin UDPGTs, isolated after amplification by PCR, shared an identical 3'-half region. Our results strongly suggest that mouse bilirubin and phenol UDPGTs are expressed from a single gene and involve alternative splicing events. We also detected duplication of the gene for phenol UDPGT in all mouse strains examined with the exception of MOL-MIT and SUB-SHH.
MeSH terms
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Animals
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Base Sequence
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Blotting, Southern
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Chromosome Mapping*
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Chromosomes, Human, Pair 1
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Gene Expression
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Genetic Linkage
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Glucuronosyltransferase / chemistry
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Glucuronosyltransferase / genetics*
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Humans
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In Situ Hybridization
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Inbred NOD
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Mice, Inbred Strains
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Molecular Sequence Data
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Polymorphism, Restriction Fragment Length
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RNA Splicing
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RNA, Messenger
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Transcription, Genetic
Substances
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DNA, Complementary
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RNA, Messenger
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bilirubin glucuronoside glucuronosyltransferase
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Glucuronosyltransferase
Associated data
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GENBANK/D50706
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GENBANK/D50707