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Fertil Steril. 1995 Oct;64(4):770-6.

Key elements of a highly efficient intracytoplasmic sperm injection technique: Ca2+ fluxes and oocyte cytoplasmic dislocation.

Author information

1
American Hospital of Paris, Neuilly sur Seine, France.

Abstract

OBJECTIVE:

To analyze the mechanism by which modifications of the intracytoplasmic sperm injection (ICSI) technique influence success rates.

DESIGN:

Prospective clinical study supplemented with an experimental analysis of Ca2+ fluxes provoked by the injection procedure.

SETTING:

Private hospital and public research center.

PATIENTS:

Patients treated by IVF and ICSI.

INTERVENTIONS:

Intracytoplasmic sperm injection.

MAIN OUTCOME MEASURES:

Fertilization and pregnancy rates and intracellular free Ca2+ concentration.

RESULTS:

The inclusion of vigorous aspiration of oocyte cytoplasm improved outcomes of ICSI. In a series of 100 consecutive cases treated with this technique, the fertilization and pregnancy rates were 87% of total metaphase II oocytes injected and 52% of total treatment cycles, respectively. Enhanced Ca2+ influx into the injected oocytes and dislocation of the oocyte cytoplasm, including the development of a focus of persistent Ca2+ discharge around the injected sperm head, were the main characteristics of this highly successful technique.

CONCLUSIONS:

Vigorous aspiration of oocyte cytoplasm may facilitate fertilization after ICSI by increasing the oocyte Ca2+ load at the time of injection, by establishing a more intimate contact of the injected sperm head with oocyte intracellular Ca2+ stores, or by a conjunction of these mechanisms.

PMID:
7672149
[Indexed for MEDLINE]

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