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Cell. 1995 Sep 8;82(5):765-71.

Calreticulin inhibits repetitive intracellular Ca2+ waves.

Author information

1
Department of Neuroscience, University of Virginia Health Sciences Center, Charlottesville 22908, USA.

Abstract

Inositol 1,4,5-trisphosphate (IP3)-mediated calcium (Ca2+) signaling is subject to cytosolic and luminal regulatory mechanisms. In Xenopus oocytes, Ca(2+)-sensitive gating of the IP3 receptor (IP3R) produces repetitive waves of Ca2+ release. We examined the role of the luminal Ca(2+)-binding protein calreticulin (CRT) in IP3-mediated Ca2+ signaling by using Ca2+ wave activity as a sensitive Ca2+ release assay. Overexpression of CRT inhibited repetitive IP3-induced Ca2+ waves. Deletion mutagenesis demonstrated that CRT inhibition was mediated by the high affinity-low capacity Ca(2+)-binding domain, which contributes little to Ca2+ storage. This novel function of CRT in intracellular Ca2+ signaling may be regulated by Ca2+ occupancy of the high affinity binding site.

PMID:
7671304
DOI:
10.1016/0092-8674(95)90473-5
[Indexed for MEDLINE]
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