Abstract
A cDNA clone encoding a taste-modifying protein, miraculin (MIR), was isolated and sequenced. The encoded precursor to MIR was composed of 220 amino acid (aa) residues, including a possible signal sequence of 29 aa. Northern blot analysis showed that the mRNA encoding MIR was already expressed in fruits of Richadella dulcifica at 3 weeks after pollination and was present specifically in the pulp.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics*
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DNA, Plant / genetics*
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Fruit / genetics
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Fruit / metabolism
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Genes, Plant
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Glycoproteins / genetics*
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Molecular Sequence Data
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sweetening Agents*
Substances
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DNA Primers
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DNA, Complementary
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DNA, Plant
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Glycoproteins
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RNA, Messenger
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Sweetening Agents
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miraculin protein, Synsepalum dulcificum