Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin

Gene. 1995 Aug 19;161(2):175-7. doi: 10.1016/0378-1119(95)00198-f.

Abstract

A cDNA clone encoding a taste-modifying protein, miraculin (MIR), was isolated and sequenced. The encoded precursor to MIR was composed of 220 amino acid (aa) residues, including a possible signal sequence of 29 aa. Northern blot analysis showed that the mRNA encoding MIR was already expressed in fruits of Richadella dulcifica at 3 weeks after pollination and was present specifically in the pulp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • DNA, Plant / genetics*
  • Fruit / genetics
  • Fruit / metabolism
  • Genes, Plant
  • Glycoproteins / genetics*
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sweetening Agents*

Substances

  • DNA Primers
  • DNA, Complementary
  • DNA, Plant
  • Glycoproteins
  • RNA, Messenger
  • Sweetening Agents
  • miraculin protein, Synsepalum dulcificum

Associated data

  • GENBANK/D38598