Format

Send to

Choose Destination
Eur J Vasc Endovasc Surg. 1995 Jan;9(1):86-92.

Shear stress induces changes in the morphology and cytoskeleton organisation of arterial endothelial cells.

Author information

1
First Department of Surgery, School of Medicine, La Sapienza University, Rome, Italy.

Abstract

OBJECTIVES:

The aim of this study was to determine the changes in the morphology and cytoskeleton organisation of endothelial cells (EC) determined by exposure to a laminar flow. Cultured EC were exposed to a wall shear stress of 6 dyne/cm2 for 24 hours.

CHIEF OUTCOME MEASURES:

The morphology of EC was analysed by light and scanning electron microscopy. The organisation of the cytoskeleton was determined by double fluorescence labeling with antibody anti-vimentin, anti-vinculin, anti-tubulin, and with rhodamine-labeled phalloidin.

RESULTS:

EC exposed to laminar flow become round-shaped with decreased area of adhesion to the substrate. There was a clear reorganisation of the cytoskeleton after exposure to shear stress; the distribution of actin changed from a stress fibre pattern to a more diffuse membrane-associated distribution. These changes in shape and cytoskeleton organisation were reversible after a 48-hour resting period.

CONCLUSIONS:

EC respond to laminar flow in a predictable manner and these findings may be correlated to the functional changes of EC observed after exposure to shear stress.

PMID:
7664019
DOI:
10.1016/s1078-5884(05)80230-8
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center