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J Struct Biol. 1995 May-Jun;114(3):177-83.

DNA at the entry-exit of the nucleosome observed by cryoelectron microscopy.

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Laboratoire d'Analyse Ultrastructurale, Section de Biologie, Université de Lausanne, Dorigny, Switzerland.


Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 on a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 +/- 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.

[Indexed for MEDLINE]

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