Format

Send to

Choose Destination
Histochemistry. 1978 Feb 3;55(1):27-40.

An improved Timm sulphide silver method for light and electron microscopic localization of heavy metals in biological tissues.

Abstract

Modifications of the Timm sulphide silver method for the demonstration of heavy metals are described. To improve the structural preservation of the tissues perfusion with a glutaraldehyde fixature is employed before perfusion with the sodium sulphide solution. For the subsequent staining for light and electron microscopy, procedures for plastic embedding, paraffin embedding and cryostat sectioning are presented. Examples from several tissues are shown, including the pituitary, pancreas, intestine, tongue, kidney, testis and brain. The staining of autolytic, postmortal human brain tissue is demonstrated.

PMID:
76622
DOI:
10.1007/bf00496691
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center