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Science. 1995 Sep 8;269(5229):1427-9.

Inducible gene targeting in mice.

Author information

1
Institute for Genetics, University of Cologne, Germany.

Abstract

A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.

PMID:
7660125
DOI:
10.1126/science.7660125
[Indexed for MEDLINE]

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