Abstract
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Crosses, Genetic
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DNA Nucleotidyltransferases / genetics
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DNA Polymerase I / genetics
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Female
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GTP-Binding Proteins*
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Gene Targeting / methods*
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Genetic Vectors
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Integrases*
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Interferon-alpha / pharmacology
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Interferon-beta / pharmacology
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Liver / drug effects
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Liver / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Transgenic
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Myxovirus Resistance Proteins
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Poly I-C / pharmacology
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Promoter Regions, Genetic
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Proteins / genetics
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Recombination, Genetic
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Sequence Deletion
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Viral Proteins*
Substances
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Interferon-alpha
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Myxovirus Resistance Proteins
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Proteins
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Viral Proteins
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Interferon-beta
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Cre recombinase
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DNA Nucleotidyltransferases
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Integrases
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DNA Polymerase I
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GTP-Binding Proteins
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Poly I-C