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Transgenic Res. 1995 Jul;4(4):288-90.

pBINPLUS: an improved plant transformation vector based on pBIN19.

Author information

1
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Molecular Biology, Wageningen, The Netherlands.

Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

PMID:
7655517
[Indexed for MEDLINE]

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