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J Vasc Res. 1995 Jul-Aug;32(4):230-6.

An in vitro study of the pharmacological responses of rat middle cerebral artery: effects of overnight storage.

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  • 1Department of Neurosurgery, Royal Hallamshire Hospital and University of Sheffield, UK.


This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alpha (PGF2 alpha), uridine triphosphate (UTP), noradrenaline (NA) and histamine were determined to investigate receptor-dependent responses, sodium nitroprusside and papaverine to investigate receptor-independent relaxation and L-arginine and N omega-Nitro-L-arginine methyl ester (L-NAME) to examine nitric oxide synthase-dependent responses. Responses in fresh arteries were established and compared with responses in arteries which had been mounted on a myograph and stored overnight at 4 degrees C before study the next day. The MCAs had an effective lumen diameter of 263 microns (SD 25) and sustained concentration-dependent contractions were produced by 124 mM K+, PGF2 alpha, UTP and L-NAME. Sodium nitroprusside, NA, histamine, papaverine and L-arginine produced concentration-dependent relaxations in precontracted arteries. Overnight cold storage did not alter the pharmacological responses to any of the agonists tested.

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