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FEMS Microbiol Lett. 1995 Aug 1;130(2-3):287-92.

Vibrio parahaemolyticus O serotypes from O1 to O13 all produce R-type lipopolysaccharide: SDS-PAGE and compositional sugar analysis.

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1
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.

Abstract

The molecular architecture of lipopolysaccharide (LPS) isolated from all O serotypes of Vibrio parahaemolyticus was investigated. In gel chromatography on a Sephadex G-50 column, the degraded polysaccharide fraction prepared from each serotype LPS by mild acid hydrolysis yielded only core oligosaccharide (Frc II) and monosaccharide (Frc III) fractions, but no fraction (Frc I) corresponding to O polysaccharide chain consisting of polymeric repeating oligosaccharide units. Compositional sugar analysis of Frc II and III suggested that the sugar chain of LPS of all the serotypes of V. parahaemolyticus consisted of at most ten monosaccharides. Sodium dodecylsulfate-polyacrylamide gel electrophoresis of the LPS resulted in no doublet ladder band similar to that observed for S-type enterobacterial LPS. These results are compatible with the interpretation that V. parahaemolyticus O serotypes from O1 to O13 all produce R-type LPS, despite the morphologically smooth appearance, demonstrated virulence and serological O-specificity.

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