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Nat Genet. 1995 May;10(1):20-7.

CpNpG methylation in mammalian cells.

Author information

1
Kanematsu Laboratories, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia.

Abstract

In vertebrate DNA, 3% to 5% of cytosine residues are present as 5-methylcytosine, and it is generally accepted that essentially all of this methylation occurs at cytosines which are contained in the symmetrical dinucleotide CpG. In this report we demonstrate, using bisulphite genomic sequencing, that the methylation machinery of mammalian cells is capable of both maintenance and de novo methylation at CpNpG sites. The existence of inherited CpNpG methylation in mammalian cells has important implications in gene regulation and in the aetiology of disease.

PMID:
7647784
DOI:
10.1038/ng0595-20
[Indexed for MEDLINE]

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