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Arch Microbiol. 1995 Jul;164(1):24-8.

Glutamate excretion in Escherichia coli: dependency on the relA and spoT genotype.

Author information

1
Forschungszentrum Jülich GmbH, Institut für Biotechnologie I, Germany.

Abstract

Glutamate excretion due to amino acid starvation was investigated in "stringent" and "relaxed" strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7-10 nmol (mg dry weight)-1 min-1. Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential.

PMID:
7646316
DOI:
10.1007/bf02568730
[Indexed for MEDLINE]

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