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Virology. 1995 Aug 20;211(2):367-76.

A defined system for in vitro lambda DNA packaging.

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1
Department of Microbiology, University of Iowa, Iowa City 52242, USA.

Abstract

We constructed a defined in vitro system for packaging lambda DNA which is composed of purified proheads, the noncapsid proteins terminase and gpFI, and the Escherichia coli DNA binding/bending protein IHF. The defined packaging system: (i) is free from endogenous ATP, DNAs, and DNases and (ii) packages 30% of the input mature lambda DNA efficiently. In this defined packaging system, IHF and gpFI make modest contributions to packaging efficiency. The defined packaging reactions showed that DNA packaging gave a linear response to the concentration of mature lambda DNA and terminase. DNA packaging showed a sigmoidal relationship with respect to the concentration of ATP and proheads.

PMID:
7645241
DOI:
10.1006/viro.1995.1419
[Indexed for MEDLINE]
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