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Gene. 1995 Jul 28;160(2):143-8.

Human DNA helicase IV is nucleolin, an RNA helicase modulated by phosphorylation.

Author information

1
International Centre for Genetic Engineering and Biotechnology, Area Science Park, Trieste, Italy.

Abstract

The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5' to 3' direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin.

PMID:
7642087
DOI:
10.1016/0378-1119(95)00207-m
[Indexed for MEDLINE]

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