Abstract
The rabbit hemorrhagic disease virus capsid protein was expressed in insect cells either as an individual protein species, from a mRNA analogous to the viral subgenomic RNA, or as part of a polyprotein that included the viral 3C-like protease and the RNA polymerase. Both pathways of expression led to the assembly of viruslike particles morphologically and antigenically similar to purified virus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Baculoviridae
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Base Sequence
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Capsid / analysis
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Capsid / biosynthesis*
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Cell Line
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DNA Primers
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DNA Probes
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Gene Expression*
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Genetic Vectors
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Hemorrhagic Disease Virus, Rabbit / genetics
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Hemorrhagic Disease Virus, Rabbit / metabolism*
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Hemorrhagic Disease Virus, Rabbit / ultrastructure
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Insecta
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Microscopy, Electron
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Molecular Sequence Data
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Polymerase Chain Reaction
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Recombinant Proteins / analysis
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Recombinant Proteins / biosynthesis
Substances
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DNA Primers
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DNA Probes
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Recombinant Proteins