Analysis of genetic structure of geographically isolated population of hybrid complex Rana esculenta L. from Danube delta according to a complex of biochemical gene markers was conducted. A parental species of Rana ridibunda and hybrids ridibunda-lessonae of the first generation only were found in the population. The complete absence of genetic recombination and introgression of genes from the locus Ldh-B, which was found in Dnieper populations, is characteristic of the population. The differences observed may be explained by genetic nonuniformity of geographical forms of R. lessonae. This forms may have taxonomic differences.