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Biochem Biophys Res Commun. 1995 Jul 26;212(3):939-44.

Alteration of transcription factor binding activities in the ischemic rat brain.

Author information

1
Division of Restorative Neurology, Baylor College of Medicine, Houston, TX 77030, USA.

Abstract

Transient focal ischemia-reperfusion in the cerebral cortex caused regional alteration of DNA-binding activities of transcription factors AP-1, CREB, Sp-1, and NF-kB. The changes were time-dependent. During the first 24 hr of reperfusion after 90 min ischemia, there was an increase in the binding activity of AP-1 only in the region surrounding the ischemic cortex. Five days after ischemia, an increase in the binding activities of CREB, Sp-1, and NF-kB, but not AP-1, was noted in the ischemic cortex, and to a lesser extent, Sp-1 and NF-kB, in the surrounding region. The binding activities of these transcription factors were reduced by hydrogen peroxide but could be restored by dithiothreitol and 2-mercaptoethanol. These results are the first demonstration of ischemia-induced differential regulation of transcription factor binding activities which are time-, region-, and redox state dependent.

PMID:
7626134
DOI:
10.1006/bbrc.1995.2060
[Indexed for MEDLINE]

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