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Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6966-70.

Expression of 5-lipoxygenase in differentiating human skin keratinocytes.

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1
Department of Internal Medicine, University of Heidelberg, Medical School, Germany.

Abstract

We studied the expression of arachidonate 5-lipoxygenase (5-LO) in a cell line of human keratinocytes (HaCaT) and in normal human skin keratinocytes in tissue culture. In undifferentiated keratinocytes 5-LO gene expression was low or undetectable as determined by 5-LO mRNA, protein, cell-free enzyme activity, and leukotriene production in intact cells. However, after shift to culture conditions that promote conversion of prokeratinocytes into a more differentiated phenotype, 5-LO gene expression was markedly induced in HaCaT cells and, to a lesser extent, in normal keratinocytes. These results show that 5-LO gene expression is an intrinsic property of human skin keratinocytes.

PMID:
7624354
PMCID:
PMC41452
[Indexed for MEDLINE]
Free PMC Article
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