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J Biol Chem. 1995 Jul 14;270(28):16549-54.

Biosynthesis of phytosiderophores, mugineic acids, associated with methionine cycling.

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1
Suntory Institute for Bioorganic Research, Osaka, Japan.

Abstract

The biosynthesis of 2'-deoxymugineic acid, a key phytosiderophore, was examined in association with the putative methionine recycling pathway in the roots of wheat using labeling experiments and structural analysis. Feeding with D-[1-13C]ribose did not result in 13C enrichment of 2'-deoxymugineic acid, while D-[2-13C]ribose resulted in 13C enrichment at the C-4", -1, -4' positions, and D-[5-13C]ribose did in C-1', -4, and -1" positions of 2'-deoxymugineic acid, respectively. Furthermore, two isotope-labeled intermediates of the methionine recycling pathway, 5-[5-2H2]methylthioribose and 2-[1-13C]keto-4-methylthiobutyric acid, were synthesized, and their incorporation into 2'-deoxymugineic acids was investigated. Six deuterium atoms at the C-4, -1', and -1" positions of 2'-deoxymugineic acid were observed after feeding with 5-[5-2H2]methylthioribose. Feeding with 2-[1-13C]keto-4-methylthiobutyric acid yielded 2'-deoxymugineic acid enriched with 13C at the C-4', -1, and -4" positions. These results demonstrated for the first time that the biosynthesis of 2'-deoxymugineic acid is associated with the methionine recycling pathway. This association system functions to recycle methionine required for continued synthesis of mugineic acids in the roots of gramineous plants.

PMID:
7622460
[Indexed for MEDLINE]
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