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Nature. 1995 Jul 20;376(6537):225-9.

Targeting of non-Ig sequences in place of the V segment by somatic hypermutation.

Author information

1
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

Abstract

Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V kappa segment can be replaced by human beta-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.

PMID:
7617031
DOI:
10.1038/376225a0
[Indexed for MEDLINE]

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