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J Clin Microbiol. 1995 May;33(5):1383-4.

Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates.

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1
Division of Infectious Diseases, Cornell University Medical College, New York, New York 10021, USA.

Abstract

We describe a rapid method for subtyping Mycobacterium tuberculosis based on PCR amplification of segments located between two distinct DNA repetitive elements. This method, double-repetitive-element PCR, classified 46 clinical isolates as having 25 distinct patterns; the conventional restriction fragment length polymorphism analysis classified the same isolates as having 23 distinct patterns. The double-repetitive-element PCR is a rapid subtyping method that has a discriminating power similar to that of the restriction fragment length polymorphism method.

PMID:
7615762
PMCID:
PMC228173
[Indexed for MEDLINE]
Free PMC Article
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