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J Cell Biochem. 1995 Apr;57(4):666-9.

Insulin receptor overexpression in 184B5 human mammary epithelial cells induces a ligand-dependent transformed phenotype.

Author information

1
Department of Medicine, University of California, San Francisco 94115, USA.

Abstract

To determine the role of the insulin receptor overexpression in breast epithelial cell transformation, the 184B5 human breast epithelial cell line was transfected with human insulin receptor cDNA. In two cell lines transfected with and overexpressing human insulin receptors (IR) (223.8 and 184.5 ng IR/10(6) cells), but not in untransfected cells, insulin binding and tyrosine kinase activity were elevated, and insulin induced a dose-dependent increase in colony formation in soft agar.

PMID:
7615651
DOI:
10.1002/jcb.240570411
[Indexed for MEDLINE]

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