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Biochem Biophys Res Commun. 1995 Jul 6;212(1):255-62.

Specific inhibitors of vacuolar type H(+)-ATPases induce apoptotic cell death.

Author information

1
Department of Oral Science, National Institute of Health, Tokyo, Japan.

Abstract

Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H(+)-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H(+)-ATPase inhibitor oligomycin and the E1E2-type H(+)-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H(+)-ATPases occurs through apoptosis.

PMID:
7612014
DOI:
10.1006/bbrc.1995.1964
[Indexed for MEDLINE]

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