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Curr Microbiol. 1995 Aug;31(2):92-6.

Targeted and random mutagenesis of the Campylobacter coli chromosome with integrational plasmid vectors.

Author information

1
Institute of Food Research, Reading Laboratory, UK.

Abstract

A number of integrational vectors were developed for use as genetic tools in the food-borne pathogen Campylobacter coli. Integration of the plasmids occurred following genetic recombination via a Campbell-like mechanism. For an integrative plasmid containing a DNA fragment internal to the C. coli catalase gene, the insertion was mutagenic and led to a catalase-deficient phenotype. A procedure for generating random mutations in the C. coli chromosome, with these suicide-plasmids, was developed. In addition, the construction and utility of an integrable plasmid for generating transcriptional fusions to a cat reporter gene is described.

PMID:
7606191
DOI:
10.1007/bf00294282
[Indexed for MEDLINE]

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