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J Appl Bacteriol. 1995 Oct;79(4):470-4.

A note on the development of a non-aldehyde fixation technique for examining spores of Bacillus subtilis under the electron microscope.

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Welsh School of Pharmacy, University of Wales College of Cardiff, UK.


Various techniques were studied for fixing spores of Bacillus subtilis prior to examining them by transmission or scanning electron microscopy. A non-aldehyde technique employing carbodiimide in cacodylate buffer produced excellent results and could be of value in studying the cytological changes produced in spores exposed to inimical treatments.

[Indexed for MEDLINE]

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