Send to

Choose Destination
FEMS Microbiol Lett. 1995 Oct 1;132(1-2):165-70.

Rapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region.

Author information

Department of Laboratory Medicine, Jikei University School of Medicine, Tokyo, Japan.


Streptococcus pneumoniae is one of the important human pathogens in clinical microbiology. A polymerase chain reaction assay was designed to detect and identify S. pneumoniae through amplification of the ribosomal DNA spacer regions between the pneumococcal 16S-23S ribosomal RNA genes. Thirty-two Streptococcus and non-Streptococcus strains were tested to verify the specificity of the assay, and only S. pneumoniae strains gave a positive reaction. This method is a powerful technique for the rapid identification of S. pneumoniae.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center