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FEBS Lett. 1995 Oct 16;373(3):269-74.

Functional expression of the human CHIP28 water channel in a yeast secretory mutant.

Author information

1
Département de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, CEA-Saclay, Gif sur Yvette, France.

Abstract

The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.

PMID:
7589481
DOI:
10.1016/0014-5793(95)01060-r
[Indexed for MEDLINE]
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