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Eur J Clin Microbiol Infect Dis. 1995 Jun;14(6):546-9.

Comparison of enzyme immunoassay antigen detection, nucleic acid hybridization and PCR assay in the diagnosis of Chlamydia trachomatis infection.

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Department of Clinical Microbiology, Tampere University Hospital, Finland.


An enzyme immunoassay (EIA) antigen detection system (MicroTrak, Syva), nucleic acid hybridization (PACE 2, Gen-Probe) and polymerase chain reaction (PCR) assay (Amplicor, Hoffmann-La Roche) were evaluated for the detection of Chlamydia trachomatis in a high-risk female population. Of 234 specimens, 42 (18%) were positive. The respective sensitivity of the EIA, RNA hybridization and the PCR was 81, 90 and 88%. When additionally performed on diluted specimens, PCR gave positive results for three of four PCR-negative specimens from EIA- and RNA-hybridization-positive women and a sensitivity of 95%. Thus, both techniques employing gene technology offered a clear improvement in sensitivity over the EIA. Future improvements in the PCR should be directed towards the elimination of polymerase inhibition.

[Indexed for MEDLINE]

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