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Eur J Clin Microbiol Infect Dis. 1995 Jun;14(6):539-42.

Comparison of two selective media and a membrane filter technique for isolation of Campylobacter species from diarrhoeal stools.

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Department of Clinical Microbiology, General Hospital Hospital Umberto 1st-Torrette, Ancona, Italy.


Diarrhoeal stool specimens from 415 patients were examined for Campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (CCDA), Skirrow medium and Columbia blood agar overlaid with a 0.65 micron pore size membrane filter. Forty-eight Campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were Campylobacter jejuni (91.7%), three were Campylobacter coli (6.3%) and one was Campylobacter hyointestinalis (2.0%). The percentages of Campylobacter-positive specimens isolated on Skirrow medium, CCDA and the membrane filter were 62, 82 and 95%, respectively. The recovery of more Campylobacter spp. from the same stool sample was achieved by the membrane filter method only. The highest isolation rate (100%) was observed when culture on CCDA and the membrane filter method were combined.

[Indexed for MEDLINE]

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