Which nutrient supplements for optimal HIV-1 production by cultured human lymphocytes?

Cell Mol Biol (Noisy-le-grand). 1995 May;41(3):423-30.

Abstract

Under standard conditions, all established cell-lines of HIV-1-infected lymphocytes are cultured in a medium containing a high percentage of serum. We have found that whilst partial or total serum depletion impedes their cell growth, it dramatically increases their virus production. Serial subcultures in serum-depleted medium are necessary to insure permanent cell growth and a relatively high reverse transcriptase activity. Following optimization of culture conditions, both HIV-infected and uninfected H9 cells have been regularly grown in a new protein-free medium. Such chemically defined culture conditions were required to detect the effect of minor components, such as vitamins, on host cell-virus interactions.

MeSH terms

  • Blood Proteins
  • Cell Division
  • Cell Line
  • Cholesterol / pharmacology
  • Culture Media*
  • Culture Media, Serum-Free
  • DNA, Viral / biosynthesis
  • HIV Reverse Transcriptase
  • HIV-1 / growth & development*
  • Humans
  • Multivariate Analysis
  • RNA-Directed DNA Polymerase / metabolism
  • Riboflavin / pharmacology
  • Time Factors
  • Virus Replication / drug effects

Substances

  • Blood Proteins
  • Culture Media
  • Culture Media, Serum-Free
  • DNA, Viral
  • Cholesterol
  • HIV Reverse Transcriptase
  • RNA-Directed DNA Polymerase
  • Riboflavin